To reverse biodiversity loss and achieve nature-positive outcomes, it is necessary for countries worldwide to adopt transformative and system-wide changes in all sectors, including the economy and business. Our global economy is dependent on nature. It also plays a big role in its degradation. With this in mind, the EU-funded SUSTAIN project will bring together a multi-stakeholder and multidisciplinary team to create a database of business dependencies and impacts. It will develop methods to reduce biodiversity-related risks, and a toolbox to support their application. By drawing on existing networks and expertise, the consortium will drive uptake of these resources through dissemination of targeted business case materials.