The Kavango-Zambezi Transfrontier Conservation Area (KAZA TFCA) in south-central Africa is the world’s largest conservation landscape. The EU-funded REWILDING project will contribute to the emerging field of environmental humanities by focusing on the shifting entanglements between people, flora and fauna in KAZA TFCA. The project will employ an ethnographic approach to understand the changing relations between humans and other species, and strive to advance knowledge about refaunation and biodiversity in conservation environments. The project’s field studies will examine how human livelihoods, institutions, social imaginaries and attitudes change under new socio-ecological situations. These field studies will feature six multispecies assemblages each comprising a loose multi-scalar network consisting of different species populations, actors, organisations, and technologies.