It is not enough to become net zero; our world needs to become nature-positive. Based on this disruptive idea, all our activities not only minimise nature loss but actually enhance nature and ecosystems. In this context, the EU-funded BIOTraCes project will develop knowledge, tools and novel approaches to enable transformative changes necessary for achieving a nature-positive society. To achieve this goal, the project will engage stakeholder networks around transformative biodiversity innovations in high-impact sectors – from agriculture and food to forestry, water and urbanisation. A series of local case studies across Europe will be used to create a portfolio of good and failed examples. A nature-positive goal is in line with the agreed global climate target of net zero emissions by 2050.